High Proliferative Placenta-Derived Multipotent Cells Express Cytokeratin 7 at Low Level
Figure 1
Characteristics of PDMCs. (a) The representative example of FISH analysis demonstrated the presence of a few maternal cells in PDMCs; for X (green) and Y (orange) chromosomes, the arrow indicates the maternal cell nucleus; ×1000. (b) A representative example of normal male karyotype of a PDMC line at P3 (n=4). (c) PDMCs were positive for CD90, CD73, CD105, and HLA-ABC at P3. (d) PDMCs were negative for CD34, CD14, CD45, and CD133 at P3. (e) Bimodal character of CD90 expression and expression of CD73 and CD105 on the CD90+ (red) and the CD90- (green) subpopulations of the PDMCs at P3. Cells stained by the corresponding antibodies are indicated by filled histograms; isotype controls are indicated by open grey histograms. (f) CK7-positive PDMCs expressed CD90 and CD105 at P3. (g) PDMCs were positive for the mesenchymal markers VIM and αSMA, and epithelial-associated proteins pCK, CK7, CGB, CK18, and CK19 at P3; brown colour – positive cells; ×50. The transcription factors CDX2, EOMES, and ERG were detected in PDMCs at P3. Scale bar: 50 μm. Because of high complexity and for better representation of multicolour confocal imaging, we only show merged images. Individual pictures of each colour channel can be obtained on request.