Baicalin Protects against Thrombin-Induced Cell Injury in Human Umbilical Vein Endothelial Cells
Figure 1
Effects of baicalin on cell survival and apoptosis after thrombin treatment. HUVECs were pretreated with baicalin (0, 50, 100, and 150μM) for 3 hours before exposure to thrombin (5 U/ml) for 6 hours. (a) Cell viability was determined by MTT assay. (b) Apoptosis was determined by flow cytometry. (c) Rate of apoptotic cells quantified of three independent experiments by flow cytometry was calculated. (d) Caspase-3 activity was determined by a caspase-3 assay kit. Caspase-3 activity relative to control that was set as 1. (e) Representative experiments of western blot for the cleaved caspase-3 protein expression. (f) Quantitative analysis of the ratio of cleaved caspase-3 to caspase-3. Values are means ± SD (n=3). ## P<0.01 vs. control group; P<0.05; P<0.01; P<0.001 vs. thrombin group.