Baicalin Protects against Thrombin-Induced Cell Injury in Human Umbilical Vein Endothelial Cells

Figure 4

Baicalin decreased thrombin-induced PAR-1 expression through inhibition of ERK1/2 phosphorylation. (a) HUVECs were pretreated with U0126 (10μM) for 3 hours before exposure to thrombin (5 U/ml). (b) HUVECs were pretreated with baicalin (50μM) for 3 hours before exposure to thrombin (5 U/ml). Representative experiments of western blot for the p-ERK1/2, ERK1/2, and PAR-1 protein expression. (A) Quantitative analysis of the ratio of p-ERK1/2 to ERK1/2, ERK1/2 was used as a loading control. (B) Quantitative analysis of the ratio of PAR-1 to GAPDH. Values are means ± SD (n=4). #P<0.05 vs. control group; P<0.05; P<0.01 vs. thrombin group.

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