Mitochondrial Division Inhibitor 1 Attenuates Mitophagy in a Rat Model of Acute Lung Injury

Figure 3

Mdivi-1 treatment inhibits LPS-induced mitochondrial dysfunction in vitro. (a) Quantification of the cells viability: the LPS + vehicle group showed remarkable decreased cell viability. Moderate (10μM) and high (50 μM) doses of Mdivi-1 treatment markedly reversed the cell viability induced by LPS exposure. (b) The cells were stained with JC-1 and the mitochondrial membrane potential (ΔΨm) was observed using laser confocal-scanning microscopy. Scale bar: 20 μM. (c) Quantification of the intracellular red and green fluorescence of JC-1. (d) Quantification of the cellular ATP levels. Data are presented as the mean ± SD (n = 6 in each group). P < 0.05 versus the control group; #P < 0.05 versus the LPS + vehicle group.

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