Biochemical and Biological Profile of Parotoid Secretion of the Amazonian Rhinella marina (Anura: Bufonidae)

Figure 4

(a) Chromatographic profile of the fraction Rm 01 (Gel Filtration) on reverse phase C18 column. The separation of the peaks C18 Rm02 and Rm 05 is highlighted by the arrows. (b) Rechromatography of the fraction C18 Rm01/02 and C18 Rm01/05. (c) 99.99 + linear gradient 0.1% ACN/TFA, 280 nm absorbance monitoring for the detection of aromatic amino acid side chains (Phenylalanine, Tyrosine, and Tryptophan). The purification profile of the sample is highlighted.