MiR-21-3p Plays a Crucial Role in Metabolism Alteration of Renal Tubular Epithelial Cells during Sepsis Associated Acute Kidney Injury via AKT/CDK2-FOXO1 Pathway
Figure 4
Effects of miR-21-3p on transcriptions and expressions of AKT and CDK2. (a) Representative results of luciferase reporter assay for AKT and miR-21-3p (: P<0.05 vs. Vector). Putative miR-21-3p binding sequence in the 3′-UTR of AKT mRNA. The mutation was generated in the AKT 3′-UTR sequence in the complementary site of the miR-21-3p seed region. (b) Representative results of luciferase reporter assay for CDK2 and miR-21-3p (: P<0.05 vs. Vector). Putative miR-21-3p binding sequence in the 3′-UTR of CDK2 mRNA. The mutation was generated in the CDK2 3′-UTR sequence in the complementary site of the miR-21-3p seed region. (c) Quantitative analysis of qRT-PCR results of AKT mRNA and CDK2 mRNA transcription levels in NRK52E cells from different groups (: P<0.05 vs. Control). (d) Representative Western-Blotting results of AKT and CDK2 expression levels in NRK52E cells from different groups (: P<0.05 vs. Control).