Function of Adipose-Derived Mesenchymal Stem Cells in Monocrotaline-Induced Pulmonary Arterial Hypertension through miR-191 via Regulation of BMPR2
Figure 2
ASCs and ASCs-Exos improved HPAECs proliferation. (a) Cell viability was analyzed by using the CCK8 assay after exposure to MCTP at concentrations of 0, 15, 30, 60, and 120 μM for 24 h. (b) Cell incubation with MCTP for 0, 12, 24, 48, and 72 h at the concentration of 60 μM. (c) Responding pictures of HPAECs cocultured with or without ASCs. Scale bars = 100 μm. (d) The number of HPAECs, counted in 3 fields under × 10 objective lens. Data are presented as mean ± SD, n =3. P < 0.01, P < 0.001 vs. HPAECs control group, #P < 0.05 vs. coculture control group. (e) Representative pictures of HPAECs cultured with or without exosomes. Scale bars = 100 μm. (f) The number of HPAECs cultured with or without exosomes, counted in 3 fields under × 10 objective lens. Data are presented as mean ± SD, n =3. P < 0.05, P < 0.001 vs. HPAECs group, #P < 0.05 vs. HPAECs+MCTP group.