GAS5 Regulates RECK Expression and Inhibits Invasion Potential of HCC Cells by Sponging miR-135b

Figure 1

Inverse correlation between expression of GAS5 and miR-135b in paired HCC samples and HCC cell lines. (a) GAS5 and miR-135b expression was detected by qRT-PCR relative to the internal control β-actin or U6B in 50 HCC tissue samples and 50 paired adjacent noncancerous liver tissue samples. (b) Correlation between GAS5 and miR-135b expressions in paired HCC samples (n=50). β-actin and U6B were served as internal controls, respectively. Statistical analysis was performed by Pearson’s correlation analysis. (c) Low expression of GAS5 (ΔΔCt<–1) associated with poor prognosis in HCC. Kaplan-Meier analysis of survival months after surgery was performed according to GAS5 expression level. (d) High expression of miR-135b (ΔΔCt>1) associated with poor prognosis in HCC. Kaplan-Meier analysis of survival months after surgery was performed according to miR-135b expression level. (e) The expression of GAS5 was lower in HCC cell lines compared with QSG-7701 normal hepatocytes assessed by qRT-PCR assay. β-actin was used as an internal control. (f) The expression of miR-135b was higher in HCC cell lines compared with QSG-7701 normal hepatocytes assessed by qRT-PCR assay. U6B was used as an internal control. (g) Correlation between GAS5 and miR-135b expressions in HCC cell lines. β-actin or U6B was served as an internal control, respectively. Statistical analysis was performed by Pearson’s correlation analysis. Data represent the mean±SD from three independent experiments. : P<0.05, : P<0.01, and : P<0.001.

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