Delivery of miRNA-29b Using R9-LK15, a Novel Cell-Penetrating Peptide, Promotes Osteogenic Differentiation of Bone Mesenchymal Stem Cells

Figure 1

Characteristics of R9-LK15/miR-29b nanocomplexes. (a) Dynamic light scattering analysis of the size and zeta potential of R9-LK15/miR-29b nanocomplexes formed by mixing R9-LK15 and miR-29b at various molar ratios. The concentration of miR-29b was 50 nM. (b) A gel retardation assay to determine the formation of nanocomplexes following mixing of R9-LK15 and miR-29b at molar ratios of 1:1–4:1. (c) A TEM image of R9-LK15/miR-29b nanocomplexes. (d) Stability of miR-29b alone and complexed with R9-LK15 upon incubation in serum at 37°C for various durations.

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