Effects of Hexane Root Extract of Ferula hermonis Boiss. on Human Breast and Colon Cancer Cells: An In Vitro and In Vivo Study
Figure 6
Light micrographs of tumor growths induced by DMBA (16 weeks postinjection) in mammary glandular tissue of female rats in comparison to rats injected with DMBA and then treated for 10 weeks with FHRH extract. (a) Proliferating neoplastic cells in the epithelial lining of mammary glandular ducts (small arrows). Neoplastic cells are also seen infiltrating the interstitial tissue. DMBA-injected animal. HE: 100X. (b) Neoplastic cell growth in the epithelial lining of mammary glandular ducts and presence of carcinomatous cells in the ductal lumina (arrowhead). DMBA-injected animal. HE: 200X. (c) Neoplastic cells in the ductal epithelial lining (arrows) and also in the ductal lumina (arrowheads). Note the nuclear pleomorphism of neoplastic cells. DMBA-injected animal. HE: 400X. (d) Area of necrosis () in the mammary glandular tissue. Note the cytoplasmic and nuclear cell debris (arrows). DMBA-injected animal. HE: 100X. (e) Restriction of the proliferating neoplastic cells to a relatively limited number of mammary glandular ducts (arrows). Note absence of distinct neoplastic cell infiltration and also absence of remarkable areas of necrosis in interstitial tissue (). FHRH extract-treated animal. HE: 400X. (f) Proliferating neoplastic cells constituting only a few layers in the epithelial lining of mammary glandular ducts (arrows). No distinct areas of necrosis in the interstitial tissue (). (g-h) Normal structure of rat mammary gland tissue. Arrow indicates the ducts and epithelial lining cells. The adipose tissue () surrounded the ducts (HE: 200x and 100x, respectively).