The Antiobesity Effects of Buginawa in 3T3-L1 Preadipocytes and in a Mouse Model of High-Fat Diet-Induced Obesity

Figure 4

Bugi inhibits the lipid accumulation and adipogenic transcription factors in eWAT. (a) The eWAT from representative mice in each group was fixed, embedded in paraffin, and stained with H&E. Images are shown at the original magnification of 100x. (b) The average diameter of adipocytes in eWAT of each group. Total RNA was also prepared from eWAT, and mRNA levels of (c) PPARγ, (d) C/EBPα, and (e) SREBP1 were assessed by quantitative RT-PCR (qRT-PCR). The values represent the mean ± S.D. of three independent experiments. ^###p < 0.001 vs. the CON group; p < 0.05, p < 0.01, and p < 0.001 vs. HFD group. Scale bar is 200 μm.

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