The Antiobesity Effects of Buginawa in 3T3-L1 Preadipocytes and in a Mouse Model of High-Fat Diet-Induced Obesity

Figure 5

Bugi inhibits the lipid accumulation and adipogenic transcription factors in liver tissue. (a) Macroscopic analysis of mouse liver tissue, and the liver tissue from representative mice in each group was fixed, embedded in paraffin, and stained with H&E. Images are shown at the original magnification of 200x. Total RNA was also prepared from liver, and mRNA levels of (b) PPARγ, (c) C/EBPα, and (d) SREBP1 were assessed by quantitative RT-PCR (qRT-PCR). The values represent the mean ± S.D. of three independent experiments. ^###p < 0.001 vs. the CON group; p < 0.001 vs. HFD group. Scale bar is 100 μm.

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