Human Embryonic Stem Cell-Derived Neural Lineages as In Vitro Models for Screening the Neuroprotective Properties of Lignosus rhinocerus (Cooke) Ryvarden

Figure 5

Effects of DEX (1 - 100 μM) on (a) neurite outgrowth of NSCs after 72 h of treatment. Results are expressed as mean ± SEM (n = 4). Asterisks denote significant differences compared to 0 μM (negative control); P < 0.01. Hashtags denote significant differences compared to B-27 treatment (positive control); ^## P < 0.01. (b) Immunostaining and fluorescence microscopy of TUJ-1 expression in NSCs treated with 0 μM, B-27 supplement and DEX (1 and 100 μM). Nuclei were stained with DAPI (blue) and neurites were stained with TUJ-1 (green). Arrows indicate neurite outgrowth that was at least twice the length of the cell body diameter. Scale bar: 100 μm.

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