Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant
In vitro plant regeneration from leaf callus (3 times subcultured) in R. tetraphylla. (a) Shoot bud induction (represented with arrows) in leaf callus cultured on MS + TDZ (0.5 mg/L). (b) Transfer of individual micro shoots on MS basal media. (c) Single shoot elongation on MS basal medium. (d) Increase in shoot length up to 1 cm on MS basal medium after 1 week of culture. (e) Increase in shoot length up to 2 cm on MS basal medium after 2 weeks of culture. (f) Root induction in single shoot cultured on MS + IAA (1mg/L) + IBA (1 mg/L). (g) Complete plantlet transferred to plastic pot containing sterile soil for hardening.