Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Table 1

Effect of 2, 4-dichlorophenoxy acetic acid (2, 4-D), on callus induction from leaf, stem (internode), and root explants of Rauwolfia tetraphylla L.

Explant	MS + 2,4-D (mg/L)	Morphogenetic Response	Callus
			Mean fresh weight (mg)	Mean dry weight (mg)
Leaf	1.0	Friable white callus	234.4 ± 0.12^i	08.2 ± 0.44^k
	2.0	Friable white callus	255.9 ± 0.23^g	09.6 ± 0.24^j
	3.0	Friable white callus	287.8 ± 0.91^f	11.4 ± 0.49^h
	4.0	Friable white callus	330.6 ± 0.76^b	16.7 ± 0.68^b
	5.0	Friable white callus	378.7 ± 0.67^a	19.2 ± 0.15^a
Stem; (Inter node)	1.0	Friable white callus	92.6 ± 0.19^l	1.03 ± 0.11^n
	2.0	Friable white callus	123.5 ± 0.28^k	2.30 ± 0.67^m
	3.0	Friable white callus	185.6 ± 0.36^j	06.6 ± 0.23^l
	4.0	Friable white callus	241.5 ± 0.34^h	12.3 ± 0.08^f
	5.0	Compact brown callus	232.2 ± 0.24^i	09.4 ± 0.24^j
Root	1.0	Friable white callus	123.6 ± 0.34^k	07.4 ± 0.55^k
	2.0	Friable white callus	256.7 ± 0.12^i	11.7 ± 0.23^g
	3.0	Friable brown callus	323.8 ± 0.36^c	15.5 ± 0.12^c
	4.0	dark brown callus	294.3 ± 0.45^d	14.6 ± 0.67^d
	5.0	dark brown callus	288.3 ± 0.44^e	13.8 ± 0.11^e

Data represents average of three replicates. Each replicate consists of 25 cultures. Data scored at the end of 3 weeks of culture. Mean ± standard error. Mean followed by the same superscript in a column is not significantly different at P=0.05.