Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Table 4

Effect of TDZ and BAP on shoot bud differentiation from 3 times subcultured leaf, stem, and root callusof Rauwolfia tetraphylla L.

Three times sub-cultured callus	TDZ; (mg/L)	BAP; (mg/L)	No. of shoot buds
Leaf	0.10	2.00	4 ± 0.1
	0.25	2.00	25 ± 0.2
	0.50	2.00	10 ± 0.1
	0.75	2.00	8 ± 0.1
	1.00	2.00	2 ± 0.1
Stem	0.10	2.00	4 ± 0.4
	0.25	2.00	20 ± 0.7
	0.50	2.00	14 ± 0.1
	0.75	2.00	4 ± 0.2
	1.00	2.00	2 ± 0.2

Data represents average of three replicates. Each replicate consists of 10 cultures. Data scored at the end of 2 weeks of culture. Mean ± standard error. Mean followed by the same superscript in a column is not significantly different at P=0.05.