Research Article
Molecular and Phenotypic Characteristics of Escherichia coli Isolates from Farmed Minks in Zhucheng, China
Table 1
Primers used in the PCRs carried out in this study.
| | Primer | Sequence (5′-3′) | Target | PCR product size (bp) |
| | Class 1 Integron | | Hep58 | TCA TGG CTT GTT ATG ACT GT | Class 1 integron variable region | Variable | | Hep59 | GTA GGG CTT ATT ATG CAC GC | | β-Lactamases | | TEM-F | ATAAAATTCTTGAAGACGAAA | blaTEM | 643 | | TEM-R | GACAGTTACCAATGCTTAATC | | SHV-F | TTATCTCCCTGTTAGCCACC | blaSHV | 860 | | SHV-R | GATTTGCTGATTTCGCTCGG | | PSE-F | TAGGTGTTTCCGTTCTTG | blaPSE | 150 | | PSE-R | TCATTTCGCTCTTCCATT | | OXA-F | TCAACTTTCAAGATCGCA | blaOXA | 591 | | OXA-R | GTGTGTTTAGAATGGTGA | | CMY-2-F | ACGGAACTGATTTCATGATG | blaCMY-2 | 714 | | CMY-2-R | GAAAGGAGGCCCAATATCCT | | Tetracyclines | | tetA-F | GCTACATCCTGCTTGCCTTC | tetA | 211 | | tetA-R | CATAGATCGCCGTGAAGAGG | | tetB-F | TTGGTTAGGGGCAAGTTTTG | tetB | 391 | | tetB-R | GTAATGGGCCAATAACACCG | | Plasmid-mediated quinolones | | qnrA-F | ATTTCTCACGCCAGGATTTG | qnrA | 519 | | qnrA-R | GATCGGCAAAGGTTAGGTCA | | qnrB-F | GATCGTGAAAGCCAGAAAGG | qnrB | 513 | | qnrB-R | ACGATGCCTGGTAGTTGTCC | | qnrC-F | GGTTGTACATTTATTGAATC | qnrC | 666 | | qnrC-R | TCCACTTTACGAGGTTCT | | qnrD-F | AGATCAATTTACGGGGAATA | qnrD | 984 | | qnrD-R | AACAAGCTGAAGCGCCTG | | qnrS-F | ACGACATTCGTCAACTGCAA | qnrS | 417 | | qnrS-R | TAAATTGGCACCCTGTAGGC | | aac(6′)-Ib-F | TTGCGATGCTCTATGAGTGGCTA | aac(6′)-Ib-cr | 482 | | aac(6′)-Ib-R | CTCGAATGCCTGGCGTGTTT | | oqxA-F | GATCAGTCAGTGGGATAGTTT | oqxA | 670 | | oqxA-R | TACTCGGCGTTAACTGATTA | | Chloramphenicols | | cmlA-F | TGCCAGCAGTGCCGTTTAT | cmlA | 900 | | cmlA-R | CACCGCCCAAGCAGAAGTA | | stcM-L | CACGTTGAGCCTCTATATGG | floR | 890 | | stcM-R | ATGCAGAAGTAGAACGCGAC | | Sulphonamides | | sul1-F | CTTCGATGAGAGCCGGCGGC | sul1 | 238 | | sul1-F | GCAAGGCGGAAACCCGCGCC | | sul2-F | GCGCTCAAGGCAGATGGCATT | sul2 | 793 | | sul2-F | GCGTTTGATACCGGCACCCGT | | sul3-R | AGATGTGATTGATTTGGGAGC | sul3 | 443 | | sul3-R | TAGTTGTTTCTGGATTAGAGCCT | | Aminoglycosides | | aac(3)-I-F | ACCTACTCCCAACATCAGCC | aac(3)-I | 528 | | aac(3)-I-R | ATATAGATCTCACTACGCGC | | aac(3)-II-F | ACTGTGATGGGATACGCGTC | aac(3)-II | 482 | | aac(3)-II-R | CTCCGTCAGCGTTTCAGCTA | | aac(3)-III-F | CACAAGAACGTGGTCCGCTA | aac(3)-III | 185 | | aac(3)-III-R | AACAGGTAAGCATCCGCATC | | aac(3)-IV-F | CTTCAGGATGGCAAGTTGGT | aac(3)-IV | 286 | | aac(3)-IV-R | TCATCTCGTTCTCCGCTCAT | | Ant(2′)-F | ATGTTACGCAGCAGGGCAGTCG | Ant(2′) | 187 | | Ant(2′)-R | CGTCAGATCAATATCATCGTGC |
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