Research Article

Inhibition of LOXL2 Enhances the Radiosensitivity of Castration-Resistant Prostate Cancer Cells Associated with the Reversal of the EMT Process

Figure 3

The radiosensitizing effect of LOXL2 knockdown in DU145 cells was associated with the reversal of the EMT phenotype and an increase in cell apoptosis. (a) The typical morphology of DU145 and LOXL2-silenced DU145 cells. (b) Protein expression of three classical EMT biomarkers, namely, E-cadherin, vimentin, and N-cadherin, was determined by western blotting. A significant reversal of the EMT process was observed in LOXL2-knockdown DU145 cells. (c-d) LOXL2 knockdown significantly enhanced cell apoptosis in DU145 cells after a 4 Gy dose of irradiation as measured by Annexin V-FITC/PI double staining. (e) LOXL2 knockdown significantly enhanced cell apoptosis in DU145 cells after a 4 Gy dose of irradiation as measured by the caspase-3 activity assay. denotes p <0.05 by Student's t-test.
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