Protective Effect of the MCP-1 Gene Haplotype against Schizophrenia

Table 1

Primers, fragment patterns, and enzymes used for RFLP analysis.

Polymorphisms	Primers (5′–3′)	Amplicon sizes (bp)	Restriction enzymes
-2518A/G (rs1024611)	F: GCTCCGGGCCCAGTATCT; R: ACAGGGAAGGTGAAGGGTATGA	182,54; 236	PvuII
362C/G (rs2857656)	F: CTAGGCTTCTATGATGCTAC; R: TCCATTCACTGCTGAGACCA	201,110; 311	Hpy188I
CCL2I/D (rs3917887)	F: GCTGATCTTCCCTGGTGCTGAT; R: CATTAAATCCCAGTGCTTCTGCCTA	I:202; D:188	—

F: forward primer; R: reverse primer; I: insertion; D: deletion. In order to ratify the data generated by the PCR-RFPL method, we analyzed 25% of the samples randomly.