Cytotoxic and Antitumor Activity of Lactaptin in Combination with Autophagy Inducers and Inhibitors

Figure 4

RL2 in combination with CQ increases percentage of dead cells. MCF-7 and MDA-MB-231 were treated with RL2 (0.15 mg/mL), CQ (20 μM), or both compounds. After 24 h of incubation, cells were collected, stained with FITC Annexin V Apoptosis detection kit (BD), and analyzed with flow cytometry. (a) The typical cell distributions in Q1-Q4 quadrants. (b) The histograms show typical cell distributions of three independent experiments.

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(b)