Adenosine Generated by Regulatory T Cells Induces CD8⁺ T Cell Exhaustion in Gastric Cancer through A2aR Pathway

Figure 1

Expression status of A2aR on CD8⁺ T cells and CD39 and CD73 on FoxP3⁺ Tregs in GC tissue. (a) Representative images of A2aR on CD8⁺ T cells and CD39 and CD73 on FoxP3⁺ Tregs, stained by 3-color tissue immunofluorescence in paired GC tissues. A2aR and FoxP3⁺ Tregs were visualized using the Cy3 channel (red), and CD8⁺ T cells and CD39 and CD73 were visualized using the FITC channel (green). DAPI was used to visualize nuclei (blue). Scale bars = 50 µm in all images. (b) Densities of CD8⁺ T cells and FoxP3⁺ Tregs and (c) ratios of A2aR⁺/CD8⁺ T cells, CD39⁺/FoxP3⁺ Tregs, and CD73⁺/FoxP3⁺ Tregs were also compared. (d) Representative images of A2aR and FoxP3 in 5 paired GC tissues by immunoblotting assays. T: cancer tissue; N: peritumoral normal tissue; n.s.: no statistical significance. ; .

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