|
| Target | Primers | Sequence (5′ to 3′) | Description |
|
| Endo-1,4-beta-glucanase | MHP168_174-F | CGCGGATCCATGTCAATATTAGAAAAAAT | Amplification |
| MHP168_174-M1R | CGCATTTCCcCAAATTCCGC | Site directed mutagenesis |
| MHP168_174-M1F | GCGGAATTTGgGGAAATGCG | Site directed mutagenesis |
| MHP168_174-R | CGCCTCGAGTTATTTATATATTAACTC | Amplification |
|
| Enolase | MHP168_271-F | CTTGGATCCATGTCAAAAATTACTAAAG | Amplification |
| MHP168_271-M1R | CTTTTTGACCATAcCAATTATCTGC | Site directed mutagenesis |
| MHP168_271-M1F | GCAGATAATTGgTATGGTCAAAAAG | Site directed mutagenesis |
| MHP168_271-M2R | GATAAAACCCTGcCAATCAGATTC | Site directed mutagenesis |
| MHP168_271-M2F | GAATCTGATTGgCAGGGTTTTATC | Site directed mutagenesis |
| MHP168_271-R | TATCTCGAGCTATTTTTTTAAGTTATAAAAAACT | Amplification |
|
| Translation elongation factor Tu | MHP168_533-F | CTCGGATCCATGGCAGTTGTTAAAACG | Amplification |
| MHP168_533-R | CCGCTCGAGTTATTTAATAATTTCGGT | Amplification |
|
| Methylmalonate-semialdehyde dehydrogenase | MHP168_244-F | CGGGGATCCATGAATTTTAAAAGAAATTAC | Amplification |
| MHP168_244-M1R | CATTTGGAATTGAcCAATTTGGCAC | Site directed mutagenesis |
| MHP168_244-M1F | GTGCCAAATTGgTCAATTCCAAATG | Site directed mutagenesis |
| MHP168_244-M2R | CGGCTTGTTTcCAAAGATCAG | Site directed mutagenesis |
| MHP168_244-M2F | CTGATCTTTGgAAACAAGCCG | Site directed mutagenesis |
| MHP168_244-M3R | GAATCTCTGAcCAGTCAATAATTG | Site directed mutagenesis |
| MHP168_244-M3F | CAATTATTGACTGgTCAGAGATTC | Site directed mutagenesis |
| MHP168_244-M4R | AATCAAATTAGTcCAGAAATTAATACTGG | Site directed mutagenesis |
| MHP168_244-M4F | CCAGTATTAATTTCTGgACTAATTTGATT | Site directed mutagenesis |
| MHP168_244-M5R | CTTCCGGGTTcCATTTTGTAG | Site directed mutagenesis |
| MHP168_244-M5F | CTACAAAATGgAACCCGGAAG | Site directed mutagenesis |
| MHP168_244-R | CTTCTCGAGTTATGACATcCAATCCTG | Amplification and site directed mutagenesis |
|
