Quantitative Proteomic Analyses of a Pathogenic Strain and Its Highly Passaged Attenuated Strain of Mycoplasma hyopneumoniae

Table 2

Proteins differentially expressed between the compared samples (M. hyopneumoniae strain 168 and strain 168L).

Identified Proteins	Accession Number	Gene_locus	Gene	ratios (168/168L)	Unique Peptide	Percentage Coverage
proteins which were more abundant in 168L
Myo-inositol catabolism protein	ADQ90457	MHP168_246	-	-3.04	10	45%
Myo-inositol 2-dehydrogenase	ADQ90458	MHP168_247	-	-2.89	16	43%
Periplasmic sugar-binding protein	ADQ90463	MHP168_252	rbsB	-2.76	21	61%
Methylmalonate-semialdehyde dehydrogenase	ADQ90455	MHP168_244	-	-2.53	21	48%
Serine hydroxymethyltransferase	ADQ90454	MHP168_243	glyA	-2.4	14	36%
5-Dehydro-2-deoxygluconokinase	ADQ90456	MHP168_245	iolC	-2.25	11	30%
Myo-inositol 2-dehydrogenase	ADQ90464	MHP168_253	-	-2.07	11	39%
Myo-inositol catabolism	ADQ90459	MHP168_248	iolD	-2.04	17	27%
Myo-inositol catabolism protein	ADQ90460	MHP168_249	iolE	-2.04	5	20%
Site-specific DNA-methyltransferase	ADQ90525	MHP168_318	mod	-1.65	7	27%
Hypothetical protein	ADQ90289	MHP168_066	-	-1.54	5	5.80%
Ribose ABC transport ATP-binding protein	ADQ90461	MHP168_250	mglA	-1.39	14	35%
Lipoprotein	ADQ90594	MHP168_392	-	-1.21	36	54%
Putative uncharacterized protein	ADQ90727	MHP168_531	-	-1.21	63	33%
Protein P97-copy 2	ADQ90409	MHP168_195	-	-1.12	34	38%
Glucose kinase	ADQ90722	MHP168_526	glcK	-1.04	7	25%
Protein p97, cilium adhesin	ADQ90328	MHP168_110	-	-1.03	70	64%
tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG	ADQ90231	MHP168_003	gidA	-1.01	12	20%
Neutrophil activating factor	ADQ90913	MHP168_736	-	-0.98	6	41%
Xylose ABC transporter ATP-binding protein	ADQ90719	MHP168_523	xylG	-0.93	15	35%
Xylose ABC transporter permease protein	ADQ90720	MHP168_524	xylH	-0.93	4	8.20%
ABC transporter ATP-binding protein	ADQ90246	MHP168_019	-	-0.91	6	8.50%
Lipoprotein	ADQ90595	MHP168_393	-	-0.88	9	28%
GTPase obg	ADQ90265	MHP168_042	obgE	-0.88	6	17%
Protein P102-copy 2	ADQ90410	MHP168_196	-	-0.83	21	25%
50S ribosomal protein L24	ADQ90339	MHP168_121	rplX	-0.81	6	48%
Heat-inducible transcription repressor hrcA	ADQ90238	MHP168_011	hrcA	-0.78	13	36%
50S ribosomal protein L23	ADQ90331	MHP168_113	rplW	-0.77	11	56%
Thioredoxin	ADQ90613	MHP168_411	-	-0.77	11	91%
Putative uncharacterized protein	ADQ90524	MHP168_317	-	-0.76	13	16%
DNA topoisomerase I	ADQ90512	MHP168_305	topA	-0.71	16	28%
50S ribosomal protein L14	ADQ90338	MHP168_120	rplN	-0.71	10	64%
Holliday junction ATP-dependent DNA helicase ruvA	ADQ90632	MHP168_430	ruvA	-0.67	8	36%
Transcription termination-antitermination factor nusA	ADQ90786	MHP168_593	nusA	-0.65	21	34%
50S ribosomal protein L3	ADQ90329	MHP168_111	rplC	-0.6	14	58%
proteins which were more abundant in 168
Triacylglycerol lipase	ADQ90483	MHP168_274	lip	0.59	13	46%
Putative uncharacterized protein	ADQ90691	MHP168_495	-	0.59	8	30%
Enolase	ADQ90480	MHP168_271	eno	0.61	14	48%
Translation elongation factor Tu	ADQ90729	MHP168_533	tuf	0.61	23	66%
Hypoxanthine phosphoribosyltransferase	ADQ90492	MHP168_283	hpt	0.61	9	50%
Transport protein sgaT	ADQ90622	MHP168_420	ulaA	0.62	7	13%
Cation-transporting P-type ATPase	ADQ90498	MHP168_289	pacL	0.63	8	13%
Hypothetical protein	ADQ90530	MHP168_323	-	0.63	5	14%
Putative uncharacterized protein	ADQ90824	MHP168_634	-	0.66	22	28%
Putative uncharacterized protein	ADQ90745	MHP168_549	-	0.68	11	46%
Acetate kinase AckA	ADQ90711	MHP168_515	ackA	0.69	17	54%
Cysteinyl-tRNA synthetase	ADQ90838	MHP168_650	cysS	0.69	7	15%
Putative uncharacterized protein	ADQ90859	MHP168_673	-	0.7	11	28%
Pts system, lichenan-specific IIa component	ADQ90264	MHP168_041	licA	0.71	9	33%
Probable O-sialoglycoprotein endopeptidase	ADQ90833	MHP168_644	gcp	0.73	10	37%
CTP synthase	ADQ90509	MHP168_302	pyrG	0.74	14	25%
Lipoate-protein ligase A	ADQ90531	MHP168_324	lplA-1	0.75	14	33%
ABC transporter ATP-binding-Pr1	ADQ90875	MHP168_691	pr1	0.75	7	16%
Hypothetical protein	ADQ90910	MHP168_731	-	0.76	4	35%
Thymidine kinase	ADQ90808	MHP168_618	tdk	0.78	8	40%
Lipoate-protein ligase A	ADQ90484	MHP168_275	lplA	0.78	5	16%
Purine-nucleoside phosphorylase	ADQ90309	MHP168_087	deoD	0.8	7	29%
Endo-1,4-beta-glucanase	ADQ90389	MHP168_174	-	0.84	20	64%
NADH dependent flavin oxidoreductase	ADQ90532	MHP168_325	baiH	0.85	4	11%
ABC transporter protein	ADQ90806	MHP168_616	-	0.87	6	11%
Sugar ABC transporter ATP-binding protein	ADQ90805	MHP168_615	-	0.9	10	26%
MG2+ ion transporter	ADQ90693	MHP168_497	mgtE	0.9	9	23%
Ribonuclease III	ADQ90602	MHP168_400	rnc	0.94	9	34%
Ribose-phosphate pyrophosphokinase	ADQ90852	MHP168_664	prsA	0.98	12	39%
Glycerol transporter subunit A	ADQ90619	MHP168_417	gtsA	1.13	4	11%
Putative uncharacterized protein	ADQ90827	MHP168_638	-	1.16	4	7.50%
hypothetical protein	ADQ90324	MHP168_104	-	1.59	19	23%
Glycerol kinase	ADQ90587	MHP168_385	glpK	1.59	22	49%
Lipoprotein	ADQ90620	MHP168_418	-	1.7	31	55%
PTS system galactitol-specific enzyme IIB component	ADQ90760	MHP168_564	-	1.78	4	57%