Research Article

Sulfatases, in Particular Sulf1, Are Important for the Integrity of the Glomerular Filtration Barrier in Zebrafish

Figure 4

Morpholino induced knockdown of sulf1, sulf2a, and sulf2b leads to reduced Vegfa expression in the glomerulus at 96 hpf. Zebrafish embryos were injected in the yolk sac at the 1-2 cell stage with either a scrambled control (CTRL) or sulf1 MO, sulf2a MO, or sulf2b MO. Fluorescence images of zebrafish larvae paraffin sections detecting WGA (red) and Vegfa (green) expression show Vegfa protein expression in the glomerulus of the pronephros in CTRL larvae. In and larvae, no expression of Vegfa is detectable in the glomerulus. larvae show a reduced Vegfa expression in the pronephric glomerulus. Scale bar = 100 μm.

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