VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells

Figure 2

VLPs containing mRNAs in a mass ratio of 6 : 1 (CP : mRNA). VLPs were run in electrophoretic shift assay. For comparison, 0.2 μg of wt-CCMV virus (lane 1) were used as control. The electrophoretic mobility of VLPs containing the capped and uncapped transcript for the EGFP is shown in lanes 2 and 3, respectively. The mRNA transcript for EGFP is shown in lane 4. The gel was stained with GelRed™, which also stains the mRNA inside the capsids, which shows that there are no free mRNA in the lanes of the VLPs. Single gel experiment without cropping.