High Expression of Acid-Sensing Ion Channel 2 (ASIC2) in Bone Cells in Osteoporotic Vertebral Fractures

Figure 4

Promotion of expression of ASICs in osteoblasts in an acidic environment. Western blots were performed to analyze ASIC1, ASIC2, ASIC3, and ASIC4 expression of osteoblasts in pH-dependent media. ASIC1, ASIC2, ASIC3, and ASIC4 were all expressed in osteoblasts. Higher expression of ASIC proteins during osteoblast differentiation in lower pH (pH 6.9) medium condition, especially more abundant levels in ASIC3 and ASIC4 expression. The most highly expressed protein at days 7, 14 and 21 was ASIC2, ASIC4, and ASIC3, respectively. p<0.05.

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