Biodegradation of Atrazine by the Novel Klebsiella variicola Strain FH-1

Table 1

Primers (F for forward and R for reverse) used for PCR amplification of Atrazine-degrading genes.

Gene	Primer	Nucleotide sequence (5′-3′)	Annealing temperature (°C)	Reference
atzA	atzA-R	TGAAGCGTCCACATTACC	50	[26]
	atzA-F	CCATGTGAACCAGATCCT
atzB	atzB-R	CTCTCCCGCATGGCATCGGG	63	[26]
	atzB-F	TCACCGGGGATGTCGCGGGC
atzC	atzC-R	GTACCATATCACCGTTTGCCA	55	[26]
	atzC-F	GCTCACATGCAGGTACTCCA
atzD	atzD-R	TGTCGGAGTCACTTAGCA	50	[29]
	atzD-F	ACGCTCAGATAACGGAGA
atzE	atzE-R	GGAGACCGGCTGAGTGAGA	50	[29]
	atzE-F	TACGCGGTAAAGAATCTGTT
atzF	atzF-R	CGATCGCCCCATCTTCGAAC	55	[29]
	atzF-F	CGATCGGAAAAACGAACCTC
trzN	trzN-R	GATTCGAACCATTCCAAACG	55	[28]
	trzN-F	CACCAGCACCTGTACGAAGG
trzD	trzD-R	GTTACGAACCTCACCGTC	50	[27]
	trzD-F	TACGCGGTAAAGAATCTGTT