Research Article

Controlled Release Strategies for the Combination of Fresh and Lyophilized Platelet-Rich Fibrin on Bone Tissue Regeneration

Table 1

The levels of growth factors released from PRF (0.2 g), lyophilized PRF (0.02 g), and PRF and lyophilized PRF in different weight ratios (F:L=1:1, 1:3, 1:5) at 1, 4, 7, 14, 21, and 28 days as determined by ELISA.

GroupAmounts of released molecules (mean and standard deviations)
D1D4D7D14D21D28

TGF-β1(ng/l)
F275.92±25.02326.80±89.32441.98±65.98329.11±48.64288.63±86.32195.75±86.59
L242.10±95.20305.34±45.03393.45±72.14407.69±115.23298.16±65.96279.11±75.36
F:L=1:1302.98±72.30293.39±42.13321.99±75.21483.92±87.25376.75±54.26231.46±68.59
F:L=1:3321.99±89.25307.69±84.26383.45±56.32312.46±76.58#240.99±81.25#198.11±65.31
F:L=1:5350.57±49.25264.82±45.32364.87±75.23398.82±86.32264.81±75.64#217.17±78.36

VEGF(ng/l)
F67.70±11.9752.01±6.9575.40±10.0744.23±10.7527.15±6.4024.37±8.46
L71.32±15.5446.05±8.88#79.63±16.7864.72±5.3823.66±11.6824.19±5.90
F:L=1:165.34±6.6164.62±6.29108.08±19.7965.89±22.6624.04±1.3423.56±8.78
F:L=1:362.27±1.9651.10±1.5272.49±3.85#73.85±4.2721.73±6.2118.27±2.51
F:L=1:561.91±6.7141.35±1.73##78.10±6.8054.23±7.1127.33±5.2517.10±2.33

PDGF-AB(ng/l)
F409.15±61.90515.27±62.91402.25±99.95426.29±8.21263.01±51.09142.05±29.53
L468.51±79.09454.97±49.66525.74±35.58396.91±79.65312.18±40.66165.41±17.79
F:L=1:1398.87±57.84487.07±59.87508.65±55.23420.23±111.51292.32±51.17127.35±24.75
F:L=1:3439.38±35.69530.56±11.78450.35±17.59458.11±22.36289.10±12.19170.27±21.59
F:L=1:5423.71±95.54432.64±40.71424.86±81.40463.30±28.30369.92±41.32119.90±16.28

Abbreviations: TGF-β1, transforming growth factor-β1; VEGF, vascular endothelial growth factor; PDGF-AB, platelet-derived growth factor. Statistically significant difference compared with F-PRF group, P < 0.01, P < 0.05, with fresh/lyophilized PRF (1:1) group, P < 0.01, P < 0.05.