Targeting Immune-Related Molecules in Cancer Therapy: A Comprehensive In Vitro Analysis on Patient-Derived Tumor Models

Figure 4

Cell cycle analysis. (a) Representative images showing apoptosis induction in Curcumin/Gemcitabine-treated HROC257 T0 M1 cells for 72 h as determined by flow cytometry and taking advantage of the BD ModFit LT Software. Cell number dependent on detected wavelength in nm (= DNA-content as an indicator for each cell cycle phase; Sub-G1: dead cells). Left: control; right: after Curcumin/Gemcitabine-treatment. (b) Quantitative cell cycle analysis of treated cell lines. Presented data result from % numbers of cells measured in each cell cycle phase (i.e., sum of G0/G1, S and M = 100%). Given are the mean values of N = 3 independent experiments. HROC, Hansestadt Rostock Colon; CIMP, CpG island methylator phenotype; MSI, microsatellite instability/instable.

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