Review Article

Nanoenabled Bioseparations: Current Developments and Future Prospects

Table 3

A summary of the bioseparations conducted using nanofibers.

Targeted BiomoleculesMembraneFunctionalizationConditions of separationType of interactionBinding capacityReference

BSA and bilirubinRC nanofiberCibacron Blue F3GA (CB)For BSA: 
(i) PBS (pH = 7.4)  
(ii) Mechanical shaking under 37°C for 3 h 
For bilirubin: 
(i) Dark room 
(ii) PBS, (pH = 8) 
(iii) Mechanical shaking under 37°C for 3 h
affinity(i) BSA: 13 mg/g  
(ii) Bilirubin: 4 mg/g
[94]

BSARC nanofiberDiethylaminoethyl (DEAE) anion-exchange ligand(i) Tris buffer (pH 8)ion exchange electrostatic(i) Static binding capacity: 40.0 mg/g  
(ii) Dynamic binding capacity at 10% breakthrough: 26.9 mg/g
[99]

Immunoglobulin G (IgG)RC nanofiberProtein A/G1: 1 (v: v)  
(A/G): PBS
affinity (covalent bonding)18 µg/mg [97]

Lipase enzyme RC nanofiberAldehyde groups(i) 4.2mg/mL NaIO4  
(ii) 6.8 h 
(iii) 30.8°C  
(iv) pH 6.1
affinity (covalent bonding)29.6 U/g lipase activity [98]

BSA and cytochrome cRC nanofiberDiethylaminoethyl (DEAE) anion-exchange ligand(i) 20 mM Bis-Tris 
(ii) 25°C 
(iii) pH 5.3
ion exchange electrostaticStatic binding capacity: 10 mg/mL [100]