Eupatilin Inhibits Renal Cancer Growth by Downregulating MicroRNA-21 through the Activation of YAP1
Figure 3
miR-21 overexpression suppresses Eupatilin-induced apoptosis and migration inhibition in renal cancer cells in vitro. (a) 786-O cells infected with miR-21 mimics or control lentivirus were treated with Eupatilin (10 mM) for 24 h. Fluorescent image of renal cancer 786-O cells showing the efficacy of lentivirus infection (Green: GFP). (b) 786-O cells infected with miR-21 mimics or control lentivirus were treated with Eupatilin (10 mM) for 24 h. The relative expression of miR-21 in 786-O cells was assessed by qRT-PCR. Data represent the mean ± SEM (n = 3) of three independent experiments. (P < 0.01 versus miR-NC; ## P < 0.01 versus miR-NC+Eupatilin). (c) 786-O cells infected with miR-21 mimics or control lentivirus were treated with Eupatilin (10 mM) for 24 h. The apoptosis of 786-O cells was detected by flow cytometry. (d) Quantification of the results in (c). Data represent the mean ± SEM (n = 3) of three independent experiments. (P < 0.01 versus miR-NC; ## P < 0.01 versus miR-NC+Eupatilin). (e) 786-O cells infected with miR-21 mimics or control lentivirus were treated with Eupatilin (10 mM) for 24 h. The migration ability of 786-O cells was detected with the Transwell assay. (f) Quantification of the results in (e). Data represent the mean ± SEM (n = 3) of three independent experiments. (P < 0.01 versus miR-NC; ## P < 0.01 versus miR-NC+Eupatilin).