Eupatilin Inhibits Renal Cancer Growth by Downregulating MicroRNA-21 through the Activation of YAP1
Figure 4
miR-21 overexpression suppresses Eupatilin-induced growth inhibition of renal cancer cells in vivo. (a) Images of nude mice injected with 786-O cells infected with miR-21 mimics or control lentivirus and tumors isolated from the mice after 35 days. Images are representative of the results obtained from six mice. (b) Mice injected with 786-O cells infected with miR-21 mimics or control lentivirus were treated with Eupatilin. After the mice were killed, tumor tissues were excised and tumor weight was measured. Data represent the mean ± SEM of six mice per group. (P < 0.01 versus miR-NC; ## P < 0.01 versus miR-NC+Eupatilin). (c) Growth curves of tumors resulting from injection of 786-O cells into nude mice. Tumor volumes were estimated using calipers. Data represent the mean ± SEM of six mice per group. (P < 0.01 versus miR-NC; ## P < 0.01 versus miR-NC+Eupatilin). (d) Nude mice injected with 786-O cells infected with miR-21 mimics or control lentivirus were treated with Eupatilin. The relative expression of miR-21 in tumors was assessed by qRT-PCR. Data represent the mean ± SEM of six mice per group. (P < 0.01 versus miR-NC; ## P < 0.01 versus miR-NC+Eupatilin).