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Figure 1: Effects of high glucose on proliferation of SCAPs. (a) Immunocytochemical staining against PBS as negative control. (b) Immunocytochemical staining against STRO-1. (c) Protein expression of RUNX2 and ALP was enhanced with the increased glucose concentrations from 5 mmol/L to 25 mmol/L, in which the highest level was detected in 25 mmol/L group. P < 0.05, P < 0.01. (d) MTT assay showed that OD values of SCAPs in 25 mmol/L group were significantly lower than those in control group at days 3, 5, and 7. Values were presented as means ± SD, n = 6, P < 0.05; P < 0.01.