mTORC2 Regulates Lipogenic Gene Expression through PPARγ to Control Lipid Synthesis in Bovine Mammary Epithelial Cells

Figure 5

RICTOR silencing decreases the expression of PPARG and LPIN1, DGAT1, ACACA and FASN in pBMECs. (a) RICTOR silencing decreased PPARG mRNA abundance (p=0.023). (b) RICTOR silencing decreased PPARγ protein level. (c) RICTOR silencing decreased the mRNA abundance of LPIN1 (p=0.025), DGAT1 (p=0.017), ACACA (p=0.022), and FASN (p=0.024). (d) RICTOR silencing decreased the protein level of intracellular PPARγ, LPIN1, ACC, and FAS. p<0.05, p<0.01, ns, no significantly; n=3 biological replicates. Error bars indicate SD.

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