Keap1 Cystenine 151 as a Potential Target for Artemisitene-Induced Nrf2 Activation
ATT-dependent Nrf2 upregulation requires functional Keap1. A549 cells (a) and WT/Keap1-knockdown MB231 cells (c) were exposed to predetermined ATT doses for 16 hours. Immunoblots show the levels of Nrf2, HO1, tubulin, and GAPDH. Control cells were treated with 50 μM tBHQ. The quantization of Nrf2 in the A549 and MB231 cells are shown in (b) and (d), respectively, (all values are represented as mean ± SD, n = 3, ).