Figure 3: Effect of 1 nM unlabeled VEGF-A on protein levels of VEGFR1 and VEGFR2. LSECs (4 × 106/ml) were treated with or without unlabeled VEGF-A (1 nM) in the presence of cycloheximide (CHX, 20 μg/ml) at 37°C for the times indicated and protein levels were analyzed by immunoblotting as described in “Materials and Methods.” (a) Shown are representative blots of two independent experiments. Molecular weight markers are indicated on the side. VEGFR2 ran as a band of approximately 230 kDa. VEGFR1 was present at approximately 180 kDa. ((b) and (c)) Pooled data from two experiments were quantified by densitometry for VEGFR1 and VEGFR2 blots and expressed as percent of untreated control after correction with α-tubulin. Data represent the mean ± range from two experiments.