Evaluation of RealStar® Alpha Herpesvirus PCR Kit for Detection of HSV-1, HSV-2, and VZV in Clinical Specimens

Table 3

Primers and probes used for the in-house developed real-time multiplex PCR assay.

Target		Sequence (5′-3′)	Amplicon size
HSV-1	Forward primer	ATCGGCGAGTACTGCATACA	75 bp (UL30 DNA polymerase gene)
	Reverse primer	GAGCTCCAGATGGGGCAA
	Probe	HEX-ATTCCCTGCTGGTGGGCCA-IABkFQ
HSV-2	Forward primer	ACGCTCTCGTAAATGCTTCC	117 bp (envelope glycoprotein G gene)
	Reverse primer	CCACCTCTACCCACAACAGA
	Probe	LC610-CGCGGAGACATTCGAGTACCAGATCG-BBQ
VZV	Forward primer	CGAGAACGGTTTGGGTTT	67 bp (ORF29 DNA binding protein gene)
	Reverse primer	CGCCGGTCGTCTCAACAG
	Probe	FAM-CGCTGCCAAGGGCCTCCTGTT-IABkFQ
Internal control	Forward primer	GTTCACCGATAGACCGCTG	113 bp
	Reverse primer	AAGAGCCCGGAATGTCAAGA
	Probe	Cy5-ACTACCTGAGCACCCAGTCCGCCCT-BBQ