Research Article
Evaluation of RealStar® Alpha Herpesvirus PCR Kit for Detection of HSV-1, HSV-2, and VZV in Clinical Specimens
Table 4
PCR results used for calculation of analytical sensitivity.
| | Target | DNA concentration (copies/reaction) | Number of replicates | Number of positives | Hit rates (%) |
| | RealStar® alpha Herpesvirus PCR assay | | HSV-1 | 6.04 × 101 | 8 | 8 | 100 | | 3.02 × 101 | 8 | 8 | 100 | | 1.51 × 101 | 8 | 8 | 100 | | 3.78 | 8 | 5 | 62.5 | | 1 | 8 | 1 | 12.5 | | No template control | 8 | 0 | 0 | | HSV-2 | 1.76 × 102 | 8 | 8 | 100 | | 8.80 × 101 | 8 | 8 | 100 | | 4.40 × 101 | 8 | 8 | 100 | | 1.10 × 101 | 8 | 5 | 62.5 | | 2.75 | 8 | 2 | 25 | | No template control | 8 | 0 | 0 | | VZV | 2.29 × 102 | 8 | 8 | 100 | | 1.15 × 102 | 8 | 8 | 100 | | 5.73 × 101 | 8 | 6 | 75 | | 1.43 × 101 | 8 | 2 | 25 | | No template control | 8 | 0 | 0 |
| | In-house multiplex PCR assay | | HSV-1 | 6.04 × 101 | 8 | 8 | 100 | | 3.02 × 101 | 8 | 8 | 100 | | 1.51 × 101 | 8 | 7 | 87.5 | | 7.55 | 8 | 2 | 25 | | 3.78 | 8 | 2 | 25 | | 1 | 8 | 0 | 0 | | No template control | 8 | 0 | 0 | | HSV-2 | 1.76 × 102 | 8 | 8 | 100 | | 8.80 × 101 | 8 | 8 | 100 | | 4.40 × 101 | 8 | 7 | 87.5 | | 2.20 × 101 | 8 | 4 | 50 | | 1.10 × 101 | 8 | 3 | 37.5 | | 2.75 | 8 | 0 | 0 | | No template control | 8 | 0 | 0 | | VZV | 2.29 × 102 | 8 | 8 | 100 | | 1.15 × 102 | 8 | 7 | 100 | | 5.73 × 101 | 8 | 6 | 75 | | 2.86 × 101 | 8 | 3 | 37.5 | | 1.43 × 101 | 8 | 0 | 0 | | No template control | 8 | 0 | 0 |
|
|
