BioMed Research International / 2019 / Article / Fig 3

Research Article

Spica Prunellae Extract Enhances Fluorouracil Sensitivity of 5-Fluorouracil-Resistant Human Colon Carcinoma HCT-8/5-FU Cells via TOP2α and miR-494

Figure 3

EESP combined with 5-FU suppressed the colony formation ability and induced apoptosis of HCT-8/5-FU cells. (a) After treatment with 5-FU (3.2 mM) with/without EESP (0.25 mg/mL or 0.5 mg/mL) or EESP (0.25 mg/mL or 0.5 mg/mL) for 48 h HCT-8/5-FU cells were fixed with methanol and stained with 0.01% crystal violet. (b) The numbers of colony formation were calculated from (a). (c) Treatment of HCT-8/5-FU cells with 5-FU (3.2 mM) with/without EESP (0.25 mg/mL or 0.5 mg/mL) or EESP (0.25 mg/mL or 0.5 mg/mL) for 48 h cell staining with annexin V/PI and analysis by using fluorescence-activated cell sorting. (d) Statistical analysis of the data from (c). Compared with the control group, , , and ; for 5-FU group, , , and .
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