Spica Prunellae Extract Enhances Fluorouracil Sensitivity of 5-Fluorouracil-Resistant Human Colon Carcinoma HCT-8/5-FU Cells via TOP2α and miR-494
EESP enhanced the 5-FU sensitivity of HCT-8/5-FU cells through inhibition of TOP2α. (a) Detection of relative protein levels for HCT-8 and HCT-8/5-FU cells treated with 5-FU (3.2 mM), with or without EESP (0.25 mg/mL or 0.5 mg/mL), for 48 h by western blot and (b) quantification. (c) Real-time PCR evaluation of the mRNA levels of TOP2A. The data from western blot and real-time PCR were normalized to GADPH. (d) Detection of levels of miR-494 by real-time PCR in HCT-8 and HCT-8/5-FU cells treated with 3.2 mM of 5-FU for 48 h (e) Relative levels of miR-494 in HCT-8/5-FU cells treated with 5-FU (3.2 mM), with or without EESP (0.25 mg/mL or 0.5 mg/mL), for 48 h. Data were normalized to U6. Compared with control, ,, and ; compared with the 5-FU (3.2 mM) treatment alone group, ,, and .
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