BioMed Research International / 2019 / Article / Fig 1

Research Article

Loratadine, an H1 Antihistamine, Inhibits Melanogenesis in Human Melanocytes

Figure 1

Effects of H1 antihistamine, loratadine, on melanogenesis in normal human melanocytes (NHM) and Mel-Ab cells. (a) NHM and Mel-Ab cells were cultured with 1.0-7.5 μM loratadine for 5 days and 4 days each and compared with 100 μg/ml arbutin. The cell images were taken using phase contrast microscopy, and the decrease in melanin is evident. (b) Loratadine suppressed tyrosinase activity in NHM and Mel-Ab cells. NHM and Mel-Ab cells were incubated with 1.0-7.5 μM loratadine for 5 days and 4 days each, and the cellular tyrosinase activity was then measured. Arbutin (100 μg/ml) was used as a positive control. Each determination was made in triplicate, and the data represent the mean ± SD. < 0.05 and < 0.01 in comparison with the untreated control. (c) The viability of NHM and Mel-Ab cells was not affected by treatment with 1.0-7.5 μM loratadine for 24 hr. Cell viability was determined using MTT assay. Each measurement was made in triplicate.
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