Lactobacillus iners Is Associated with Vaginal Dysbiosis in Healthy Pregnant Women: A Preliminary Study

Table 1

Species-specific primer sets for detection of vaginal bacteria by quantitative real-time PCR.

PCR specificity	Primer	Sequence (5′-3′)	Annealing temp.	Amplicon size (bp)	Reference
All bacteria	Bac27F	AGAGTTTGATCCTGGCTCAG	65°C	312	[28]
	EUB338R-I	GCTGCCTCCCGTAGGAGT
Lactobacillus genus	Bact-0011	AGAGTTTGATYMTGGCTCAG	62°C	667	[29]
	Lab-0677	CACCGCTACACATGGAG
L. crispatus	Lcris-F	AGCGAGCGGAACTAACAGATTTAC	65°C	154	[30]
	Lcris-R	AGCTGATCATGCGATCTGCTT
L. jensenii	Ljens-F	AAGTCGAGCGAGCTTGCCTATAGA	60°C	162	[31]
	Ljens-R	CTTCTTTCATGCGAAAGTAGC
L. iners	Liners-F	CTCTGCCTTGAAGATCGGAGTGC	65°C	155	[31]
	Liners-R	ACAGTTGATAGGCATCATCTG
Atopobium vaginae	AV-F	TAGGTCAGGAGTTAAATCTG	62°C	156	[32]
	AV-R	TCATGGCCCAGAAGACCGCC
Gardnerella vaginalis	GV1-F	TTACTGGTGTATCACTGTAAGG	62°C	332	[33]
	GV3-R	CCGTCACAGGCTGAACAGT
Eggerthella	Egger-621F	AACCTCGAGCCGGGTTCC	60°C	239	[34]
	Egger-859R	TCGGCACGGAAGATGTAATCT
Leptotrichia/Sneathia	Lepto-395F	CAATTCTGTGTGTGTGAAGAAG	60°C	252	[34]
	Lepto-646R	ACAGTTTTGTAGGCAAGCCTAT
Megasphaera type I	MegaE-456F	GATGCCAACAGTATCCGTCCG	64°C	212	[34]
	MegaE-667R	CCTCTCCGACACTCAAGTTCGA
Prevotella	Prevo-F	CCAGCCAAGTAGCGTGCA	60°C	151	[35]
	Prevo-R	TGGACCTTCCGTATTACCGC