Effect of Chemotherapeutics and Tocopherols on MCF-7 Breast Adenocarcinoma and KGN Ovarian Carcinoma Cell Lines In Vitro

Figure 3

Effect of αToc on cell viability. MCF-7 and KGN cells were exposed to αToc 0, 50, 75, 100μM for 24h exposure (24H+), 24h exposure and 24h culture with media (24H+24H-), 24h exposure and 48h culture with media (24H+48H-), or 72h continuous exposure (72H+). Culture media containing 0.8% DMSO was used as a control. Cell viability was assessed by a crystal violet assay, in which cell number was obtained by comparison with a standard curve and % cell viability was calculated from vehicle control. Means ± SD of 3 independent experiments shown. Data analysed by one-way ANOVA with Tukey’s post hoc test.

(a) MCF-7 cells

(b) KGN cells