Effect of Chemotherapeutics and Tocopherols on MCF-7 Breast Adenocarcinoma and KGN Ovarian Carcinoma Cell Lines In Vitro

Figure 6

Effect of chemotherapeutics and tocopherols on estrogen production. KGN cells were exposed to Dox (0, 5, 10, 25μM), 4-Cyc (0, 0.5, 1, 2.5μM), αToc (0, 50, 75, 100μM) or γToc (0, 50, 75, 100μM) for 24h exposure (24h+), 24h exposure and 24h culture with fresh DMEM/F-12 complete medium (24h+24h-), 24h exposure and 48h culture with DMEM/F-12 complete medium (24h+48h-), or 72h continuous exposure where reagents in medium + 10% FCS were replenished every 24h (72h+). Estrogen production was assessed in supernatant at the end of each exposure using an estradiol Enzyme-Linked Immunoassay, in which concentration of estrogen (pg/mL) was obtained by comparison with a standard curve, and estrogen/cell concentration was calculated by dividing pg/mL of estrogen by the number of viable cells in the same well. Means ± SD of 3 independent experiments shown. Data analysed by one-way ANOVA with Tukey’s post hoc test. p ≤ 0.05; p ≤ 0.01, p ≤ 0.0001 compared to the same exposure control.

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