Effect of Chemotherapeutics and Tocopherols on MCF-7 Breast Adenocarcinoma and KGN Ovarian Carcinoma Cell Lines In Vitro

Figure 7

Effect of chemotherapeutics and tocopherols on estrogen production. Combined chemotherapeutic regime (25μM Dox with 2.5μM 4-Cyc), combined regime + 75μM αToc, or combined regime + 75μM γToc for 24h exposure (24h+), 24h exposure and 24h culture with fresh DMEM/F-12 complete medium (24h+24h-), 24h exposure and 48h culture with DMEM/F-12 complete medium (24h+48h-), or 72h continuous exposure where reagents in medium + 10% FCS were replenished every 24h (72h+). Estrogen production was assessed in supernatant at the end of each exposure by using an estradiol ELISA, in which concentration of estrogen (pg/mL) was obtained by comparison with a standard curve, and estrogen/cell concentration was calculated by dividing pg/mL of estrogen by the number of viable cells in the same well. Means ± SD of 3 independent experiments shown. Data analysed by one-way ANOVA with Tukey’s post hoc test. p ≤ 0.05; p ≤ 0.01, p ≤ 0.0001 compared to control same concentration of doxorubicin alone (10μM).