Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from Corneal Scrapings

Figure 1

First and second round of PCR amplifications (a) Uniplex PCR with ITS1 & ITS4 primers: Lane 1 - 100 bp ladder, lane 2 - Fusarium sp. (∼600 bp), lane 3 - A. flavus, lane 4 - A. fumigatus, lane 5 - Bipolaris spp., lane 6 - Exerohilum sp., lane 7 - Alternaria sp., lane 8 - Curvularia sp. (b) Multiplex PCR with species specific primers: Lane 1 - 100 bp marker, Lane 2 - Fusarium sp., Lane 3 - A. flavus, Lane 4 - A. fumigatus, Lane 5 - Bipolaris sp., Lane 6 - Exerohilum sp., Lane 7 - Alternaria sp., Lane 8 - Curvularia sp. Lane 9 - UID, Lane 10 - UID.

(a)

(b)