Antinociceptive Activity of Petroleum Ether Fraction of Clinacanthus nutans Leaves Methanolic Extract: Roles of Nonopioid Pain Modulatory Systems and Potassium Channels

Figure 2

Effect of PECN on glutamate–induced nociception in mice. Animals were treated with vehicle (10 mL/kg, p.o.), ASA (100 mg/kg, p.o.), or PECN (100, 250, 500 mg/kg, p.o.) 60 min before intraplantar administration of glutamate (10 umol/paw prepared in normal saline; 20 μL) into the right hind paw. Each column represents the mean ± SD of six mice. Statistical analyses were performed using 1–way ANOVA followed by Bonferroni’s post hoc test. < 0.05 significantly differed when compared to control group; < 0.05 significantly differed when compared to ASA; < 0.05 data with different superscript differed significantly when compared together. Values in parentheses denote percentage of inhibition.