BioMed Research International / 2019 / Article / Fig 3

Research Article

TRPV1 Induced Apoptosis of Colorectal Cancer Cells by Activating Calcineurin-NFAT2-p53 Signaling Pathway

Figure 3

Increased cytosolic Ca2+ influx and NFAT protein induced by TRPV1 overexpression. (a) Measurement of Ca2+ influx by staining with Fluo-4 AM in HCT116 cells following treated with capsaicin (50 μM) and then detected by using flow cytometer. (b) The expression levels of p-NFAT2 and NFAT2 protein were examined by Western blotting, and the relative intensity was shown as a bar graph. (c) The expression level of NFAT2 protein was examined by immunofluorescence assay; images were observed by fluorescence microscopy (magnification, ×400). The result was shown as means ± standard deviation. p < 0.05 and p < 0.01.

(a)
(b)
(c)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.