TRPV1 Induced Apoptosis of Colorectal Cancer Cells by Activating Calcineurin-NFAT2-p53 Signaling Pathway

Figure 3

Increased cytosolic Ca²⁺ influx and NFAT protein induced by TRPV1 overexpression. (a) Measurement of Ca²⁺ influx by staining with Fluo-4 AM in HCT116 cells following treated with capsaicin (50 μM) and then detected by using flow cytometer. (b) The expression levels of p-NFAT2 and NFAT2 protein were examined by Western blotting, and the relative intensity was shown as a bar graph. (c) The expression level of NFAT2 protein was examined by immunofluorescence assay; images were observed by fluorescence microscopy (magnification, ×400). The result was shown as means ± standard deviation. p < 0.05 and p < 0.01.

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