A Seamless Gene Deletion Method and Its Application for Regulation of Higher Alcohols and Ester in Baijiu Saccharomyces cerevisiae

Figure 5

PCR verification of mutant strains Hα5+H (50, 150, 300, 700, 1000). M DL5000 DNA marker; lanes 1-lanes 6 PCR verification results from mutant strains Hα5+H (50), Hα5+H (150), Hα5+H (300), Hα5+H (700), Hα5+H (1000), parental strain, respectively, using primer pairs AHB-A-U and AHB-A-D; lanes 7-lanes 12 PCR verification results from mutant strains Hα5+H (50), Hα5+H (150), Hα5+H (300), Hα5+H (700), Hα5+H (1000), parental strain, respectively, using primer pairs AHB-B-U and AHB-B-D.