The Reproducibility of the Immunohistochemical PD-L1 Testing in Non-Small-Cell Lung Cancer: A Multicentric Italian Experience

Table 1

Technical protocols in gold standard and open platforms of the study.

PROTOCOLS	CLONE	PLATFORM	TITER	PH	Incubation primary Ab	Incubation linker/polymer
Gold standard 1	22C3	Autostainer	PharmDx prediluted	low; 20′-97°	30′	30′/30′
Gold standard 2	SP263	Ventana	prediluted	(CC1) High pH ETDA retrieval buffer	16′- 37°	-
1	28-8	Dako Omnis	1:500	high; 30′-97°C	20′	10′/20′
2	22C3	Dako Omnis	1:20	low; 40′ - 97°	40′	10′/40′
3	22C3	Dako Omnis	1:20	low; 40′ - 97°	40′	10′/40′
4	22C3	Dako Omnis	1:50	low; 30′ - 97°	30′	10′/30′
5	22C3	Dako Omnis	1:50	low; 30′ - 97°	30′	10′/30′
6	22C3	Dako Omnis	1:20	low; 40′ - 97°	40′	20′/40′
7	22C3	Ventana	1:40	(CC1) High pH ETDA retrieval buffer	32′ - 36°	12′ with OptiView DAB IHC detection kit